Isolation and characterization of chicken b-catenin
نویسندگان
چکیده
b-catenin interacts with a number of proteins in di erent important biological processes, including cell adhesion through cadherins, actin organization through fascin, body axis determination through Wnt signaling, tumor suppression through APC, and transcriptional activation through LEF-1. To examine its function in chicken embryogenesis, we isolated the chicken homolog of b-catenin from a chicken embryo cDNA library. The sequence is highly conserved at the amino acid level between chicken, mouse (99%), human (99%) and Xenopus (97%). In-situ hybridization and immunostaining showed that in the developing limb, it is specifically expressed in the apical ectodermal ridge, suggesting a role in epithelial–mesenchymal interactions. © 1997 Elsevier Science B.V.
منابع مشابه
Isolation and characterization of mycoflora of chicken hatcheries in Mazandaran province, north of Iran
Fungal infections cause significant economic losses in the poultry industry either due to their direct infectious nature or due to mycotoxins production. Hatchery contamination with fungi can threaten chicken health. In this regard, geographical and seasonal distributions of airborne fungal contamination of 25 hatcheries in Mazandaran province, northern Iran, were investigated using an open pla...
متن کاملGenetic Characterization of Toxoplasma gondii Isolated from Chicken Meats in Tunisia
Background: Toxoplasmosis is one of the most important food-borne diseases distributed worldwide. In order to determinate the involvement of poultry meat in clinical toxoplasmosis, this study aimed to genetic characterization of Toxoplasma gondii in tissue samples of serologically positive chickens of Tunisia using Polymerase Chain Reaction (PCR). Methods: Trail of isolation of local T. gondii...
متن کاملIsolation and identification of Salmonellae from chicken carcasses in processing plants in Yazd province, central Iran
This study was conducted to determine the prevalence of Salmonellae contamination of chicken carcasses in slaughterhouses in central Iran (Yazd province). 435 samples were obtained from liver, breast— before and after chilling—and bacteriological and serological examinations were done. The results showed that the rate of contamination of liver, breast meat—before and after chiller—were 8.1, 18....
متن کاملIsolation, identification and antimicrobial sensitivity of Ornithobacterium rhinotracheale in broilers chicken flocks of Khuzestan, Iran
Ornithobacterium rhinotracheale (ORT) is a bacterium associated with respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. The objective of this study was isolation, identification and evaluation of antimicrobial susceptibility of ORT bacterium in slaughtered broilers chicken flocks based on cultural and molecular tests in Khuzestan pr...
متن کاملDetection and Molecular Characterization of Sorbitol Negative Shiga Toxigenic Escherichia Coli in Chicken from Northwest of Iran
Shiga toxin-producing Escherichia coli (STEC) are food-borne pathogens primarily associated with the consumption of contaminated ground beef and are an important food safety concern worldwide. STEC has been found to produce a family of related cytotoxins known as Shiga toxins (Stxs). Shiga toxins have been classified into two major classes, Stx1 and Stx2. A single strains of STEC can produce St...
متن کامل